Quantification of active components in dermatological formulations, especially, Hydroquinone, Tretinoin and Salicylic Acid is also an important element of pharmaceutical quality control. These two ingredients are usually used together to treat skin diseases including hyperpigmentation, acne, and psoriasis because of the complementary properties. This review will examine the current developments in the Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) methods of simultaneous analysis of the three actives. The main aspects to be focused on are column choice, mobile phase optimization, wavelengths of detection and outcomes of method validation. The development of Ultra High-Performance Liquid Chromatography (UHPLC) which has been observed has greatly accelerated speed and sensitivity of analysis through the use of smaller particle sizes and faster run time and the development of advanced detectors like Photodiode Array (PDA) detectors has also enhanced the ability to detect several compounds simultaneously. Moreover, there is a move in trends towards green chromatography and automation which is making the methods of analysis increasingly sustainable and efficient. However, issues like complexity of matrices, co-elution and degradation of actives are present and thus there is still need to develop more on stability-indicating techniques and measuring all the three actives simultaneously in one technique. This review highlights the importance of these innovations towards enhancing the effectiveness, safety, and regulatory conformance of dermatological procedures.